Sequencing activites for the characterization of viral genomes

Available Analyses

Manual, semi-automated, and automated methods are used for viral genome sequencing :

ANALYSIS	INDICATION	METHOD	REAGENTS
Genotyping of HBV	Clinical research Molecular epidemiology	Sequencing the HBsAg coding region	CNR Protocol
Genotyping of HBV resistance to antiviral drugs	Characterization of treatment failure of nucleoside/nucleotide analogues	Sequencing the reserve transcriptase coding region	CNR Protocol
Determining the genotype and sub-type of HCV	Pre-therapeutic assessment of HCV	Hybridization on a strip	Siemens ref.6719382
Genotyping of HCV resistance to antiviral drugs	Characterization of treatment failure based on the use of direct acting antiviral drugs	Sequencing the regions coding the proteins NS3, NS5A ans NS5B	CNR Protocol
Genotyping of HIV resistance to antiviral drugs	Characterization of treatment failure of antiretroviral drugs	ViroSeq	Abbott ref.4J9493
Characterization of the sequence of integrase	Approach to therapeutic decision Characterization of integrase inhibitors in treatment failure	ViroSeq	Abbott ref.4J9471

Equipment

Materials used for viral genome sequencing are shared between the Laboratory of Virology and the genomic platform [Sanger and Next Generation sequencing (NGS)]. The equipment includes: