Viral serology activities

Viral serology activities involve virus detection, quantifying viral antigens, and confirming the presence of virus or specific antibodies targeted against the virus, confirming contact with the pathogen. Viral serology can be used for screening, diagnosis, or monitoring infections, as well as to assess immunity, acquired naturally or after vaccination.

Available virological markers

The Laboratory of Virology can detect and, if necessary, quantify many serological markers of viral infection. The available technologies are based on immuno-enzymatic methods, such as manual or automated ELISA (enzyme-linked immunosorbent assay). Techniques based on immunoblotting (western blot) can also be used for certain markers.

Automated immuno-enzymatic methods (Ag: antigen)

ANALYSIS	INDICATION	DEVICE	REAGENTS
HBsAg qualitative	Diagnosis of HBV Therapeutic follow-up	Vitros 3600	Ortho ref.6802131
HBsAg qualitative		Vidas	Biomérieux ref.30315
HBsAg quantitative		Architect	Abbott ref.6C36-42
anti-HBc		Vitros 3600	Ortho ref.8496812
anti-HBc		Vidas	Biomérieux ref.30314
anti-HBs	Measurement of HBV antibodies (vaccination card)	Vitros 3600	Ortho ref.1787753
anti-HBs	Measurement of HBV antibodies (vaccination card)	Vidas	Biomérieux ref.30238
IgM anti-HBc	Diagnosis of acute Hepatitis B	Vitros 3600	Ortho ref.8577413
IgM anti-HBc	Diagnosis of acute Hepatitis B	Vidas	Biomérieux ref.30349
HBeAg	Classification of chronic Hepatitis B	Vidas	Biomérieux ref.30305
anti-Hbe	Classification of chronic Hepatitis B	Vidas	Biomérieux ref.30305
anti-HCV	Diagnosis of Hepatitis C	Vidas	Biomérieux ref.30308
anti-HCV	Diagnosis of Hepatitis C	Vitros 3600	Ortho ref.1318450
HAV total antibodies	Measurement of HAV antibodies (vaccination card)	Vitros 3600	Ortho ref.6801461
HAV total antibodies	Measurement of HAV antibodies (vaccination card)	Vidas	Biomérieux ref.30312
IgM anti-HAV	Diagnosis of acute Hepatitis A	Vitros 3600	Ortho ref.8450124
IgM anti-HAV	Diagnosis of acute Hepatitis A	Vidas	Biomérieux ref.30307
p24 antigen + HIV 1+2 antibodies	HIV Diagnosis	Architect	Abbott ref.4J27
p24 antigen + HIV 1+2 antibodies	HIV Diagnosis	Vidas	Biomérieux ref.30443
p24 antigen	Primary HIV Diagnosis	Vidas	Biomérieux ref.30117
HTLV 1+2 antibodies	Diagnosis of HTLV 1	Architect	Abbott ref.6L61-25
HTLV 1+2 antibodies	Diagnosis of HTLV 1	Liaison XL	Diasorin ref.310270
CMV IgG antibodies	Diagnosis of CMV	Liaison XL	Diasorin ref.310745
CMV IgG antibodies		Vidas	Biomérieux ref.30204
CMV IgM antibodies		Liaison XL	Diasorin ref.310755
CMV IgM antibodies		Vidas	Biomérieux ref.30205
EBV VCA IgG antibodies	Diagnosis of EBV infection	Liaison XL	Diasorin ref.310510
EBV VCA IgG antibodies		Vidas	Biomérieux ref.30236
EBV VCA IgM antibodies		Liaison XL	Diasorin ref.310500
EBV EBNA IgG antibodies		Liaison XL	Diasorin ref.310520
EBV EBNA IgG antibodies		Vidas	Biomérieux ref.30235
IgG rubella antibodies	Rubella Diagnosis	Vidas	Biomérieux ref.30221
IgM rubella antibodies	Rubella Diagnosis	Vidas	Biomérieux ref.30214
HSV antibobies	Detection of antibodies to HSV-1 and HSV-2	Liaison XL	Diasorin ref.310800
VZV antibodies	Detection of antibodies to varicella zoster virus	Vidas	Biomérieux ref.30217
Parvovirus B19 IgG antibodies	Primary diagnosis of parvovirus B19	Liaison XL	Diasorin ref.317000
Parvovirus B19 IgM antibodies	Primary diagnosis of parvovirus B19	Liaison XL	Diasorin ref.317010

Manual immuno-enzymatic methods :

ANALYSIS	INDICATION	REAGENTS
Adeno-Rota virus antigen combo test, human stool specimens	Etiological diagnosis of acute gastroenteritis	Rbiopharm ref.N1003
HIV-1/-2 antibody differentiation	Diagnosis of a primary infection	Alere ref.60432002
Hepatitis E virus IgG antibody test (RDT*)	Diagnosis of acute Hepatitis E	Nephrotek ref.43160020

*RDT: rapid diagnostic test

Other methods :

ANALYSIS	INDICATION	METHOD	REAGENTS
anti-HDV	Diagnosis of Hepatitis Delta	ELISA	Diasorin ref.P2808
p24 antigen	Diagnosis of primary HIV infection	ELISA	Internat ref.80563
p24 antigen neutralisation	Confirmation of the diagnosis of primary HIV infection	ELISA	Internat ref.80565
HIV-1 antibody confirmation	Confirmation of the diagnosis of HIV-1 infection	Western Blot	Biorad ref.72251
HIV-2 antibody confirmation	Confirmation of the diagnosis of HIV-2 infection	Western Blot	Biorad ref.72252

Equipment

The laboratory of virology has several automated systems that allow the detection and quantification of viral antigens and antibodies against viruses:

1 VIDAS (BioMérieux)

2 VIDAS 3 (BioMérieux)

1 LIAISON XL (DiaSorin)

1 VITROS 3600 (Ortho)

1 ARCHITECT (Abbott)

VHC - Virus Hépatologie Cancer | HENRI MONDOR

Viral serology activities

Available virological markers

Equipment