VHC - Virus Hépatologie Cancer | HENRI MONDOR

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Detection and quantification
of viral genomes

 

Detection, generally associated with the quantification of viral genomes (DNA or RNA of the virus), is an essential element for direct diagnosis in virology. Indeed, the presence of the viral genome is an indication of viral multiplication at the site of the targeted organ. The methods of detection and quantification of viral genomes are principally based on amplification of the target (PCR for “polymerase chain reaction” or TMA for “transcription-mediated amplification”) or amplification of the signal (bDNA for “branched DNA”).

Available Biological Analyses

Detection and quantification of viral RNAs and DNAs are realized using automated systems :

ANALYSIS

INDICATION

DEVICE

REAGENTS




Detection/quantification of HBV ADN (quantification of viral load)



Diagnosis and moritoring of chronic hepatitis B

Therapeutic follow-up of chronic hepatitis B


GeneXpert


Cepheid ref.MADGXHBV-VL-CE-10


Cobas


Roche ref.4894570190


m2000


Abbott ref.2G3490





Detection/quantification of HCV RNA (quantification of viral load)


Diagnosis and moritoring of  acute or chronic hepatitis C



Therapeutic follow-up of chronic hepatitis C


m2000


Abbott ref.4J8690


GeneXpert


Cepheid ref.MADGXHCV-VL-CE-10


Cobas


Roche ref.5532264190



Detection/quantification of HIV RNA (quantification of viral load)


Diagnosis of HIV infection


Therapeutic follow-up of HIV infection


Cobas


Roche ref.5212294190


GeneXpert


Cepheid ref.MADGXHIV-VL-CE-10


Detection/quantification of HEV RNA (quantification of viral load)


Diagnosis and moritoring of acute or chronic hepatitis E


m2000


Altona ref.271013




Detection/quantification of CMV DNA in whole blood (quantification of viral load)


Diagnosis of primary CMV infection

Diagnosis of CMV reactivation

Therapeutic follow-up of CMV infection





Rotor-Gene Q





Qiagen ref.4503363



Detection/quantification of EBV DNA in whole blood (quantification of viral load)


Diagnosis of EBV reactivation

Diagnosis of EBV-associated lymphoproliferation

Therapeutic follow-up of EBV infection





Rotor-Gene Q





Qiagen ref.4501363


Detection/quantification of BK virus DNA in urine sample or whole blood (quantification of viral load)


Moritoring of kidney transplant
Etiological diagnosis of hemorrhagic cystitis



Rotor-Gene Q



Qiagen ref.4514363


Detection/quantification of HHV-6DNA in  whole blood (quantification of viral load)


Etiological diagnosis of fevers or encephalitis of an immunocompromised host



Rotor-Gene Q



Elitech ref.RTS036PLD


Qualitative detection of HSV-1 and HSV-2 DNA


Etiological diagnosis of encephalitis (HSV-1) and meningitis (HSV-2)



StepOnePlus



Biomérieux ref.69-004B


Qualitative detection of VZV DNA


Etiological diagnosis of vesicular legions Etiological diagnosis of viral meningitis



StepOnePlus



Biomerieux ref.69-004B


Qualitative detection of Enterovirus DNA


Etiological diagnosis of viral meningitis


GeneXpert


Cepheid ref.MADGXEV-100N-10



Qualitative detection of HHV-6 DNA except for whole blood (other samples)


Etiological diagnosis of fevers or encephalitis of an immunocompromised host



Rotor-Gene Q



Elitech ref.RTS036PLD



Qualitative detection of HHV-8 DNA


Etiological diagnosis of Kaposi's sarcoma, serous lymphoma, Castleman's disease




Rotor-Gene Q




Elitech ref.RTS038PLD




Qualitative detection of parvorirus B19 DNA


Diagnosis of primary parvovirus B19 infection

Etiological diagnosis of anemia in immunocompromised patiens
Etiological diagnosis of anemia in patients with hemoglobinopathies





Rotor-Gene Q





Elitech ref.RTS070PLD



Qualitative detection of adenovirus DNA

Etiological diagnosis of gastroenteritis and viral resembling pneumonia

Etiological diagnosis of severe infections in immunocompromised patients




StepOnePlus




Biomérieux ref.69-010B


Qualitative detection of zika virus RNA


Etiological diagnosis of a virus from an at-rick area


Rotor-Gene Q


Altona ref.591013


Multiple qualitative detection of genomes of respiratory viruses : respiratory syncytial virus, human metapneumovirus, rhinovirus/enterovirus, adenovirus, coronavirus, parainfluenza, influenza A (H1N1, H3N2) et B viruses



Etiological diagnosis of acute pneumonia



Filmarray




Biomérieux
ref. RFIT-ASY-0136

 


Qualitative detection of influenza A virus RNA (flu)







Etiological diagnosis of acute pneumonia


GeneXpert


Cepheid ref.MADGXFLU/RSV-CE-10


Qualitative detection of influenza B virus RNA (flu)


GeneXpert


Cepheid ref.MADGXFLU/RSV-CE-10


Qualitative detection of respiratory syncytial virus RNA


GeneXpert


Cepheid ref.MADGXFLU/RSV-CE-10


Norovirus qualitative PCR


Suspected viral Gastroenteritis


GeneXpert


Cepheid ref.MADGXNOV-CE-10

 

Equipment

The Laboratory of Virology is equipped with several automated systems designed to detect and quantify viral genomes:

  • 1 PANTHER real-time PCR extractor/thermocycler (Hologic)
  • 1 COBAS AmpliPrep automatic nucleic acids extractor (Roche Molecular Systems) coupled with 1 COBAS Taqman 96 real-time PCR thermocycler (Roche Molecular Systems)
  • 1 m2000SP automatic nucleic acids extractor (Abbott Molecular) coupled with 1 m2000RT real-time PCR thermocycler (Abbott Molecular)
  • 3 QIAsymphony Extractor SP/AS (Qiagen)


  •  1 ABI7300real-time PCR thermocycler (Applied Biosystems)
    • 2 StepOnePlus real-time PCR thermocyclers (Applied Biosystems)
    • 2 Rotor-Gene Q thermocyclers (Qiagen)
    • 1 Filmarray (BioMérieux)
    • 2 ABI GENEAMP 2700 thermocycler (Applied Biosystems)
    • 2 Veriti thermocycler (Applied Biosystems)