PhD, PU-PH, PharmD,
stephane.chevaliez@aphp.fr
The activities of the NRC concerning viral hepatitis B, C, and delta are divided into three complementary axes that cover the fields of hepatitis virology, from technological investigation to clinical and translational research.
These axes are:
Technological expertise
Several evaluations of virological diagnostic tests for HCV and HBV have been conducted in partnership with the manufacturers who developed them. They include: molecular biology techniques applied to the detection and/or quantification of HCV RNA and HBV DNA by real time PCR or TMA; and techniques for typing viral genomes by reverse hybridization or sequencing to identify viral genotype and subtype or resistance mutations after nucleotide sequence analysis (direct sequencing methods, high throughput sequencing). The development of alternative diagnostic methods (rapid diagnostic orientation tests) on blood samples for screening and diagnosis of viral hepatitis is in full swing.
The kits evaluated during 2016 are presented in the table below:
Kit | Application | Manufacturer |
Abbott RealTime HCV |
| Abbott |
Cobas Taqman HCV v2.0 | Roche | |
Architect HCV Ag | Abbott | |
OraQuick HCV test |
| OraSure |
First Response HCV Card | Premier Med Corp | |
Assure HCV |
| |
MultiSure HCV | ||
OraQuick HCV test | OraSure | |
Toyo HCV |
| |
Labmen HCV | ||
VERIS HBV | Detection-quantification of HBV DNA | Beckman |
Aptima HCV Quant Dx | Detection-quantification of HCV RNA | Hologic |
Aptima HBV Quant | Detection-quantification of HBV DNA | Hologic |
Molecular epidemiology
The virology laboratory, in collaboration with the INSERM research group, has developed all the tools for molecular typing and genome analysis of HBV and HCV (PCR, cloning, classical and high throughput sequencing, and genetic and phylogenetic analyses) needed for the virology component of many national epidemiological surveys in association with SpF.
Examples of the studies are presented in the table below:
Study or Investigation | Main objective |
Prevagay 2015 | Seroprevalence of HIV and hepatitis B and C of MSM who frequent places for socializing. |
BaroTest | National survey of hepatitis B and C and HIV by self-collection. |
Afrobaromètre Santé | Prevalence of hepatitis B and C in African and Caribbean populations in the Ile de France. |
Clinical and translational research
Genotypic and phenotypic studies of hepatitis virus resistance to antivirals
Performance of rapid diagnostic tests for the detection of anti-HBs in various patient populations
J Clin Virol. 2017
The New Aptima HCV Quant Dx Real-time TMA Assay Accurately Quantifies Hepatitis C Virus Genotype 1-6 RNA
J Clin Virol. 2017
Dried Blood Spots: A Tool to Ensure Broad Access to Hepatitis C Screening, Diagnosis, and Treatment Monitoring
J Infect Dis. 2016
New virologic tools for management of chronic hepatitis B and C
Gastroenterology 2012
Evaluation of a new random-access HBV DNA molecular assay: The VERIS HBV assay
J Clin Virol. 2017
Approaches for simplified HCV diagnostic algorithms
JIAS 2017