VHC - Virus Hépatologie Cancer | HENRI MONDOR

Soutenir la recherche

Organization and resources

The French National Reference Center for Viral Hepatis B, C, and delta (NRC) was created in January 2006, under the direction of Professor Jean-Michel Pawlotsky, following the mandate of the previous NRC, headed by Professor Christian Bréchot, in the context of a reorganization of activities between the coordinating and associated laboratories.
The NRC is housed within the Laboratory of Virology of the Department of "Virology, Hygiene, Bacteriology, Parasitology-Mycology, Transversal unit of Infection Treatment" of the Henri Mondor Medical Center and relies on the skills and expertise of this laboratory, as well as the research group " Physiopathology and Therapeutics of Chronic Viral Hepatitis and related Cancers" (INSERM U955) with which it is affiliated.

General Organization

The NRC, which performs both medical and scientific activities, has multiple connections, relationships, and collaborations with institutional bodies:

  • The French National Public Health Agency [merging the French Institute for Health Promotion and Health Education (Inpes), French Institute for Public Health Surveillance (InVS), and the Establishment for Public Health Emergency Preparedness and Response (Eprus) as of May 1, 2016]

The NRC also cooperates with the public and private sectors involved in surveillance or associated with epidemiology within the framework of their activities (hospitals, clinics, medical biology analysis laboratories, research laboratories, etc.).

Human resources

Medical personnel assigned to the NRC:

  • Dominique CHALLINE, PH (Director)
  • Stéphane CHEVALIEZ, PU-PH
  • Jean-Michel PAWLOTSKY, PU-PH
  • Christophe RODRIGUEZ, MCU-PH

Technical staff assigned to the NRC:

  • Nazim AHNOU, Research Engineer
  • Magali FREDERIC, Medical-Technical Services Manager
  • Sabah HAMADAT, Research Engineer
  • Valérie ORTONNE, Senior Technician
  • Lila POITEAU, Senior Technician
  • Alexandre SOULIER, Senior Hospital Engineer
  • Secretary

IT Infrastructure

The computer system of the laboratory meets the requirements of a medical biology laboratory that hosts sensitive data, in accordance with article L.1111-8 of the Public Health Code. The computer system consists of a GLIMS laboratory management system and a MEDIWEB results server. These systems meet the standard requirements that guarantee the confidentiality of computerized patient information. The IT infrastructure and related systems are protected by the Information Systems Department (DSI). The DSI ensures: (1) protection of the computer system against computer piracy, especially during remote maintenance, (2) automatic and daily backup of data, and (3) access to patients results according to regulations.

Equipment and logistics

The staff, expertise, equipment of the laboratory of virology, next Generation Sequencing (NGS) platform, and the INSERM U955 research group are available to the NRC for it to carry out its missions.

The following equipment is available to the NRC:

Automated viral serology platform

  • 3 VIDAS 3 (BioMérieux)
  • 1 LIAISON XL (DiaSorin)
  • 1 VITROS 3600 (Ortho)
  • 1 ARCHITECT (Abbott)


Automated devices used for the detection and quantification of nucleic acids

  • 1 PANTHER TMA PCR extractor/thermocycler (Hologic)
  • 1 m2000SP automated nucleic acid extractor (Abbott)
  • 3 QIAsymphony SP/AS extractors (Qiagen)

  • 1 m2000RT thermocycler for RealTime PCR (Abbott)
  • 1 ABI7300 thermocycler for RealTime PCR (Applied Biosystems)
    • 2 ABI GENEAMP 2720 thermocyclers (Applied Biosystems)
    • 2 Veriti thermocyclers (Applied Biosystems)

    Automated devices used for the characterization of viral genome sequences

    • 1 AUTOBLOT 3000 automated reverse hybridization system (Siemens)
    • 1 Auto-LiPA 48 (Innogenetics)

    Materials used for the constitution and conservation of libraries

    • Laminar flow hoods
    • -80°C Freezers
    • Cold room and -20°C freezers
    • Liquid nitrogen tanks