VHC - Virus Hépatologie Cancer | HENRI MONDOR

Soutenir la recherche

Associated laboratory : Hepatitis Delta


The NRC's "hepatitis delta" laboratory for viral hepatitis B, C, and delta is located in the Virology Unit of the Clinical Microbiology Laboratory of the Paris Seine-Saint-Denis University Hospitals - UFR SMBH, Université Paris Nord (Bobigny). It is directed by Dr. Emmanuel Gordien.


  • Ensure the prospective typing of all new strains of hepatitis delta virus (HDV) circulating in France.
  • Evaluate and develop diagnostic tests for serological screening and the detection and quantification of HDV RNA in various biological tissues to facilitate the management of patients infected with this virus.
  • Ensure the management and scientific valorization of the NRC-D (collection of plasma and serum samples and complementary RNA and DNA).
  • Organize annual national quality control for the diagnosis of HDV infection by serological and molecular biology tests.
  • Strengthen its role as a reference laboratory at the national and international level

Human resources

Medical personnel assigned to the associated laboratory HDV:

  • Ségolène BRICHLER, MCU-PH
  • Alloui CHAKIB, PH
  • Emmanuel GORDIEN, MCU-PH

Technical staff assigned to the associated laboratory HDV:

  • Zahia BEN ABDESSELAM, clinical research associate
  • Samira DZIRI, Senior technician
  • Athenaïs GERBER, Research Engineer
  • Frédéric LE GAL, Research Engineer
  • Secretary

Available virological markers

The following techniques are available at the hepatitis delta NRC:

  • Serological techniques for the detection of total anti-delta antibodies (ETI-AB-DELTAK-2, Sorin Biomedica), anti-delta IgM antibodies (ETI-DELTA-IGMK-2, Sorin Biomedica), and delta antigen (ETI-DELTAK-2 Sorin Biomedica)
  • Detection and quantification of HDV RNA by quantitative plasma RT-PCR
  • Qualitative detection of HDV RNA
  • Characterization of the HDV genotype
  • Complete sequencing of the HDV genome

Sample procedures

Exam request should be accompanied by an information sheet duly completed and sent to the following address:


Download Fact Sheet

The associated laboratory HDV accepts the following types of samples : decanted and frozen serum, decanted and frozen plasma. For all other types of samples, please contact the NRC (Tel: 33148955611 / Fax : 33148955911 / mail : emmanuel.gordien@aphp.fr).

Research topics

The group is interested in the diversity of circulating hepatitis delta viruses and the consequences of this diversity on viral infection and pathogenesis. The research topics developed are:

  • The epidemiology of patients infected with HDV, associated clinical diseases, modes of transmission, treatment, and prevention
  • The molecular mechanisms of HDV replication.
  • The epidemiology of patients infected with HDV, associated clinical diseases, modes of transmission, treatment, and prevention.

The research of the laboratory has led to a new Delta virus classification system. Four new HDV genotypes have been characterized in the laboratory, such that HDV can now be classified into eight distinct genetic groups (or clades, or genotypes) all descended from a common ancestor (Radjef N., et al., 2014 and Le Gal F et al., 2016). This work led to the filing of an international patent on behalf of the Assistance Publique-Hôpitaux de Paris, "HDV Nucleic Acid Molecules, Their Fragments and Applications" (PCT/FR02/03239), in September 2001. The NRC has now characterized more than 2,500 HDV sequences.

In 2005, the laboratory developed the first consensus method for quantifying RNA viral load by real-time RT-PCR. This "in-house" technique was the subject of a patent application ("Quantification of VHD viral load by real-time PCR" - PCT/FR02/03239) in September 2005.