Sample Processing

Sample Procedures

The French National Reference Center for Viral Hepatitis B, C, and delta (NRC) is open during normal business hours on working days. However, samples can be accepted 7 days a week, 24 hours a day (24/7) at the welcome desk of the Laboratory of Virology at the Henri Mondor Hospital.

Exam requests should be accompanied by an information sheet duly completed and sent to the following address:

CNR DES HEPATITES VIRALES B, C ET DELTA
DÉPARTEMENT DE VIROLOGIE, BACTERIOLOGIE-HYGIENE, PARASITOLOGIE-MYCOLOGIE
GROUPE HOSPITALIER HENRI MONDOR
51 AVENUE DU MARECHAL DE LATTRE DE TASSIGNY
94010 CRETEIL
FRANCE

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Download Fact Sheet - DBS

The NRC accepts the following types of samples: decanted and frozen serum, decanted and frozen plasma, frozen RNA or DNA extracts, and room temperature DBS. For all other types of samples, please contact the NRC.

Available virological markers

The NRC has several techniques for the diagnosis and identification of viruses, as well as typing, to assess sensitivity or resistance to antivirals.

Automated immuno-enzymatic methods :

	ANALYSIS	INDICATION	DEVICE	REAGENTS
Sera/ Plasma	HBsAg qualitative	Diagnosis of HBV Therapeutic follow-up	Vitros 3600 (Ortho)	Ortho, ref.6802131
	HBsAg qualitative		Vidas (Biomerieux)	Biomerieux, ref.30315
	HBsAg quantitative		Architect (Abbott)	Abbott, ref.6C36-42
	HBsAg quantitative		Vidas (Biomerieux)	Biomérieux, ref.30317
	anti-HBc		Vitros 3600 (Ortho)	Ortho, ref.849 6812
	anti-HBc		Vidas (Biomerieux)	Biomerieux, ref.30314
	anti-HBs	Measurement of HBV antibodies (vaccination card)	Vitros 3600 (Ortho)	Ortho, ref.178 7753
	anti-HBs	Measurement of HBV antibodies (vaccination card)	Vidas (Biomerieux)	Biomérieux, ref.30238
	IgM anti-HBc	Diagnosis of acute Hepatitis B	Vitros 3600 (Ortho)	Ortho, ref.857 7413
	IgM anti-HBc	Diagnosis of acute Hepatitis B	Vidas (Biomerieux)	Biomerieux, ref.30439
	HBeAg	Classification of chronic Hepatitis B	Vidas (Biomerieux)	Biomerieux, ref.30305
	anti-Hbe	Classification of chronic Hepatitis B	Vidas (Biomerieux)	Biomerieux, ref.30305
	anti-VHD	Diagnosis of Hepatitis Delta	ELISA Manuel	Diasorin, ref.P2808
	anti-VHC	Diagnosis of Hepatitis C	Vidas (Biomerieux)	Biomerieux, ref.30308
	anti-VHC	Diagnosis of Hepatitis C	Vitros 3600 (Ortho)	Ortho, ref.131 8450
	VHC capsid Ag	Clinical research	Architect (Abbott)	Abbott, ref,6L47-27
DBS	HBsAg qualitative	Diagnosis of HBV	Reagents not validated for blotting paper
	HBsAg quantitative	Diagnosis of HBV
	anti-VHC	Diagnosis of Hepatitis C

Quantification and sequencing activities for the characterization of viral genome :

	ANALYSIS	INDICATION	DEVICE/METHOD
Sera/ Plasma	Detection/quantification of HBV DNA (quantification of viral load)	Diagnosis and monitoring of chronic hepatitis B Therapeutic follow-up	Cobas TaqMan (Roche)
			m2000 (Abbott)
	HBV Genotyping	Clinical research Molecular epidemiology	Sequencing of the HBsAg coding region
	Genotyping of HBV resistance to antiviral drug	Characterization of treatment failure of nucleoside/nucleotide analogues	Sequencing of the RT coding region
	Assessment of PreC/C drug-associated substitutions		Sequencing of the PreC/C coding region
	Assessment of PreS/S drug-associated substitutions		Sequencing of the PreS/S coding region
	Detection/quantification of HCV RNA (quantification of viral load)	Diagnosis and monitoring of acute or chronic hepatitis C Therapeutic follow-up	m2000 (Abbott)
			GeneXpert (Cepheid)
			Cobas TaqMan (Roche)
	Determination of HCV genotype and sub-type	Pre-therapeutic assessment of HCV	Sequencing of the region encoding the protein NS5B
			Sequencing of the region encoding the protein E1
			Hybridization on a strip (Lipa)
	Genotyping of HCV resistance to antiviral drugs	Characterization of treatment failure based on the use of direct-acting antiviral drugs	Sequencing of the regions encoding the proteins NS3, NS5A and NS5B
	HCV coinfection suspicion	Suspicion of treatment failure/coinfection	Sequencing of the regions encoding the proteins E1, HRV1 and NS5B
DBS	Detection/quantification of HCV RNA (quantification of viral load)	Treatment evaluation	Reagents not validated for blotting paper
DBS	Determination of HCV genotype and sub-type	Treatment evaluation	Sequencing of the region encoding the protein NS5B

VHC - Virus Hépatologie Cancer | HENRI MONDOR

Sample Processing

Sample Procedures

Available virological markers